plastic piece printed 2d bar code Search Results


pieceid-2''-o-gallate  (TargetMol)
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cryovials 96 well format external thread screw cap vial with 2d barcode and jacket  (FluidX Inc)
90
FluidX Inc cryovials 96 well format external thread screw cap vial with 2d barcode and jacket
Cryovials 96 Well Format External Thread Screw Cap Vial With 2d Barcode And Jacket, supplied by FluidX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cryovials 96 well format external thread screw cap vial with 2d barcode and jacket - by Bioz Stars, 2026-09
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nupage bis tris gel  (Thermo Fisher)
99
Thermo Fisher nupage bis tris gel
(A) In vitro translation was directed by a truncated proOmpA 1-143 (Met1 to Thr143) message that was followed by a codon for one of 20 amino acids . Reaction was allowed at 37°C for 30 min in the presence of [ 35 S]methionine. Macromolecules were precipitated with trichloroacetic acid (final concentration, 5%) and the precipitates were washed twice with acetone and dissolved in 2 x Laemmli sample buffer (see for the composition) that had been treated with RNASecure (Ambion), at room temperature followed by incubation at 37°C for 5 min. Samples were then divided into 3 portions. The first sample was untreated. The second and the third samples were mixed with an equal volume of 0.4 M Tris-base and incubated at 70°C for 15 and 30 min, respectively. They were separated by <t>NuPAGE</t> to visualize 35 S-labeled proOmpA 1-143 -X-tRNA bands with phosphor imaging. Numbers indicate intensities of the bands, after incubation with Tris-base, relative (%) to that of the untreated sample. (B) Stability of proOmpA 1-143 -Val-tRNA and proOmpA 1-143 -His-tRNA was examined as described in (A) except that protein precipitates were dissolved in 1 x sample buffer, to which 1/10 volume of either 2 M Na 2 CO 3 or 2 N NaOH was added and incubated at 37°C or 70°C for 15 min as indicated. (C) Stability of proOmpA 1-143 -Val-tRNA and proOmpA 1-143 -His-tRNA was examined as described in (A) except that samples were mixed with an equal volume of either 0.4 M, 1.0 M or 2.0 M Tirs-base and incubated at 80°C for 20 min as indicated.
Nupage Bis Tris Gel, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plastic+piece+printed+2d+bar+code/TRIS-HCL/pmc03230602-142-15-21
Average 99 stars, based on 1 article reviews
nupage bis tris gel - by Bioz Stars, 2026-09
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imprinted piece of polydimethylsiloxane (pdms)  (Corning Life Sciences)
90
Corning Life Sciences imprinted piece of polydimethylsiloxane (pdms)
(A) In vitro translation was directed by a truncated proOmpA 1-143 (Met1 to Thr143) message that was followed by a codon for one of 20 amino acids . Reaction was allowed at 37°C for 30 min in the presence of [ 35 S]methionine. Macromolecules were precipitated with trichloroacetic acid (final concentration, 5%) and the precipitates were washed twice with acetone and dissolved in 2 x Laemmli sample buffer (see for the composition) that had been treated with RNASecure (Ambion), at room temperature followed by incubation at 37°C for 5 min. Samples were then divided into 3 portions. The first sample was untreated. The second and the third samples were mixed with an equal volume of 0.4 M Tris-base and incubated at 70°C for 15 and 30 min, respectively. They were separated by <t>NuPAGE</t> to visualize 35 S-labeled proOmpA 1-143 -X-tRNA bands with phosphor imaging. Numbers indicate intensities of the bands, after incubation with Tris-base, relative (%) to that of the untreated sample. (B) Stability of proOmpA 1-143 -Val-tRNA and proOmpA 1-143 -His-tRNA was examined as described in (A) except that protein precipitates were dissolved in 1 x sample buffer, to which 1/10 volume of either 2 M Na 2 CO 3 or 2 N NaOH was added and incubated at 37°C or 70°C for 15 min as indicated. (C) Stability of proOmpA 1-143 -Val-tRNA and proOmpA 1-143 -His-tRNA was examined as described in (A) except that samples were mixed with an equal volume of either 0.4 M, 1.0 M or 2.0 M Tirs-base and incubated at 80°C for 20 min as indicated.
Imprinted Piece Of Polydimethylsiloxane (Pdms), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plastic+piece+printed+2d+bar+code/imprinted+piece+of+polydimethylsiloxane++pdms+/pmc05940309-223-21-29
Average 90 stars, based on 1 article reviews
imprinted piece of polydimethylsiloxane (pdms) - by Bioz Stars, 2026-09
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matrix tube  (Thermo Fisher)
95
Thermo Fisher matrix tube
(A) In vitro translation was directed by a truncated proOmpA 1-143 (Met1 to Thr143) message that was followed by a codon for one of 20 amino acids . Reaction was allowed at 37°C for 30 min in the presence of [ 35 S]methionine. Macromolecules were precipitated with trichloroacetic acid (final concentration, 5%) and the precipitates were washed twice with acetone and dissolved in 2 x Laemmli sample buffer (see for the composition) that had been treated with RNASecure (Ambion), at room temperature followed by incubation at 37°C for 5 min. Samples were then divided into 3 portions. The first sample was untreated. The second and the third samples were mixed with an equal volume of 0.4 M Tris-base and incubated at 70°C for 15 and 30 min, respectively. They were separated by <t>NuPAGE</t> to visualize 35 S-labeled proOmpA 1-143 -X-tRNA bands with phosphor imaging. Numbers indicate intensities of the bands, after incubation with Tris-base, relative (%) to that of the untreated sample. (B) Stability of proOmpA 1-143 -Val-tRNA and proOmpA 1-143 -His-tRNA was examined as described in (A) except that protein precipitates were dissolved in 1 x sample buffer, to which 1/10 volume of either 2 M Na 2 CO 3 or 2 N NaOH was added and incubated at 37°C or 70°C for 15 min as indicated. (C) Stability of proOmpA 1-143 -Val-tRNA and proOmpA 1-143 -His-tRNA was examined as described in (A) except that samples were mixed with an equal volume of either 0.4 M, 1.0 M or 2.0 M Tirs-base and incubated at 80°C for 20 min as indicated.
Matrix Tube, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plastic+piece+printed+2d+bar+code/Matrix+2D+Barcoded+Open-Top+Storage+Tubes/us09416394-384-31-34
Average 95 stars, based on 1 article reviews
matrix tube - by Bioz Stars, 2026-09
95/100 stars
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pieceid-2''-o-gallate  (Biosynth Carbosynth)
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Image Search Results


(A) In vitro translation was directed by a truncated proOmpA 1-143 (Met1 to Thr143) message that was followed by a codon for one of 20 amino acids . Reaction was allowed at 37°C for 30 min in the presence of [ 35 S]methionine. Macromolecules were precipitated with trichloroacetic acid (final concentration, 5%) and the precipitates were washed twice with acetone and dissolved in 2 x Laemmli sample buffer (see for the composition) that had been treated with RNASecure (Ambion), at room temperature followed by incubation at 37°C for 5 min. Samples were then divided into 3 portions. The first sample was untreated. The second and the third samples were mixed with an equal volume of 0.4 M Tris-base and incubated at 70°C for 15 and 30 min, respectively. They were separated by NuPAGE to visualize 35 S-labeled proOmpA 1-143 -X-tRNA bands with phosphor imaging. Numbers indicate intensities of the bands, after incubation with Tris-base, relative (%) to that of the untreated sample. (B) Stability of proOmpA 1-143 -Val-tRNA and proOmpA 1-143 -His-tRNA was examined as described in (A) except that protein precipitates were dissolved in 1 x sample buffer, to which 1/10 volume of either 2 M Na 2 CO 3 or 2 N NaOH was added and incubated at 37°C or 70°C for 15 min as indicated. (C) Stability of proOmpA 1-143 -Val-tRNA and proOmpA 1-143 -His-tRNA was examined as described in (A) except that samples were mixed with an equal volume of either 0.4 M, 1.0 M or 2.0 M Tirs-base and incubated at 80°C for 20 min as indicated.

Journal: PLoS ONE

Article Title: Nascentome Analysis Uncovers Futile Protein Synthesis in Escherichia coli

doi: 10.1371/journal.pone.0028413

Figure Lengend Snippet: (A) In vitro translation was directed by a truncated proOmpA 1-143 (Met1 to Thr143) message that was followed by a codon for one of 20 amino acids . Reaction was allowed at 37°C for 30 min in the presence of [ 35 S]methionine. Macromolecules were precipitated with trichloroacetic acid (final concentration, 5%) and the precipitates were washed twice with acetone and dissolved in 2 x Laemmli sample buffer (see for the composition) that had been treated with RNASecure (Ambion), at room temperature followed by incubation at 37°C for 5 min. Samples were then divided into 3 portions. The first sample was untreated. The second and the third samples were mixed with an equal volume of 0.4 M Tris-base and incubated at 70°C for 15 and 30 min, respectively. They were separated by NuPAGE to visualize 35 S-labeled proOmpA 1-143 -X-tRNA bands with phosphor imaging. Numbers indicate intensities of the bands, after incubation with Tris-base, relative (%) to that of the untreated sample. (B) Stability of proOmpA 1-143 -Val-tRNA and proOmpA 1-143 -His-tRNA was examined as described in (A) except that protein precipitates were dissolved in 1 x sample buffer, to which 1/10 volume of either 2 M Na 2 CO 3 or 2 N NaOH was added and incubated at 37°C or 70°C for 15 min as indicated. (C) Stability of proOmpA 1-143 -Val-tRNA and proOmpA 1-143 -His-tRNA was examined as described in (A) except that samples were mixed with an equal volume of either 0.4 M, 1.0 M or 2.0 M Tirs-base and incubated at 80°C for 20 min as indicated.

Article Snippet: The gel piece was then placed on the top of the second dimension gel (12% NuPAGE Bis-Tris gel for 2D from Invitrogen) and overlaid with NuPAGE stacking gel solution ( http://openwetware.org/wiki/Sauer:bis-Tris_SDS-PAGE%2C_the_very_best , except that acrylamide concentration was 5%) followed by its polymerization.

Techniques: In Vitro, Concentration Assay, Incubation, Labeling, Imaging